Thyroglobulin-specific t cell line from a healthy individual does not produce proinflammatory cytokines on antigenic stimulation: an implication for possible fail-safe mechanism to avoid autoimmunity
作者:
ShimojoNaoki,
KatsukiToshiyuki,
SaitoKimiyuki,
TarutaniOsamu,
KohnoYoichi,
NiimiHiroo,
期刊:
Autoimmunity
(Taylor Available online 1994)
卷期:
Volume 17,
issue 3
页码: 203-207
ISSN:0891-6934
年代: 1994
DOI:10.3109/08916939409010655
出版商: Taylor&Francis
关键词: thyroglobulin;Healthy;T cell clone;Cytokine;fail-safe mechanism
数据来源: Taylor
摘要:
In order to investigate the regulation of autoimmune response to thyroglobulin (Tg), one of the thyroid autoantigens, we established a Tg-specific T cell line by stimulation of peripheral blood mononuclear cells from a healthy volunteer with Tg and characterized its cytokine production pattern. The Tg-specific Tcell line, designated DH5D1, obtained from a limiting dilution culture bore ap”Tcell receptor and was CD4 and CD45RO positive. Upon stimulation with Tg, DH5D1 secreted little or no titers of IL-2, TNF-a, and IFN-γ, whereas activation with combination of phorbor myristate acetate and calcium ionophore produced measurable levels of these cytokines. These results indicate that the Tg-specific T cell line is not defective in its capacity to produce proinflammatory cytokines and suggest that the inability of cytokine production by autoreactive T cells of healthy individuals is one fail-safe mechanism for preventing aggression of harmful autoimmune response.
点击下载:
PDF (442KB)
返 回