High Performance Liquid Chromatographic Determination of Amiodarone in Plasma and Tissues
作者:
A.A. Al-Dhawailie,
期刊:
Analytical Letters
(Taylor Available online 1995)
卷期:
Volume 28,
issue 13
页码: 2391-2400
ISSN:0003-2719
年代: 1995
DOI:10.1080/00032719508000380
出版商: Taylor & Francis Group
关键词: Amiodarone;HPLC;Plasma;Tissue
数据来源: Taylor
摘要:
A simple and rapid high-performance liquid chromatographic (HPLC) method for the determination of amiodarone (AD) in plasma and tissues was developed. The method involved deproteinization of plasma or homogenized tissue with acetonitrile containing an internal standard (N-Cetylpyridinium chloride) followed by reversed phase chromatography using μ bondapack C18column (10μm) with a mobile phase consisting of acetonitrile - methanol - sodium dihydrogen phosphate buffer (70:10:20%, v/v), the pH adjusted to 4.0 and pumped at flow rate of 1.0 ml/min. The column effluent was monitored at 242 nm. A linear relationship was obtained between peak height ratios (drug to internal standard) versus drug levels over the concentration range of 50–750 ng/ml. The detection limit of AD in plasma and tissues by this method was 20 ng/ml.
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