The effect of bleomycin on DNA synthesis in ataxia telangiectasia lymphoid cells
作者:
Maimon M. Cohen,
Stacy J. Simpson,
期刊:
Environmental Mutagenesis
(WILEY Available online 1982)
卷期:
Volume 4,
issue 1
页码: 27-36
ISSN:0192-2521
年代: 1982
DOI:10.1002/em.2860040105
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: ataxia telangiectasia;lymphoblastoid cell lines;DNA synthesis;inhibition;bleomycin;x‐rays;radiomimetics
数据来源: WILEY
摘要:
AbstractBleomycin, a radiomimetic glycopeptide, inhibits de novo DNA synthesis in ataxia telangiectasia lymphoblastoid B cells to a markedly lesser extent than in normal and xeroderma pigmentosum lymphoid cells. This observaton is similar to that following ionizing radiation; however, the effect is slower following the chemical treatment. Recovery of the normal cells occurs 15–18 hours after treatment, whereas the ataxia telangiectasia lines do not attain normal levels of DNA synthesis during the entire 24‐hour observation period. Similar differences were not observed following treatment with mitomycin C, a bifunctional alkylating agent, indicating a specific effect of bleomycin on DNA synthesis in ataxia telangiectasia cells. Following bleomycin treatment and preincubation with hydroxyurea, residual DNA synthesis in ataxia telangiectasia cells was similar to that in both normal and xeroderma pigmentosum lymphoid lines, suggesting that the capacity to repair the induced DNA lesion is pres
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