The effects of cationic hexadecyltrimethylammonium chloride (CTAC), nonionic polyoxyethylene (9.5)p-1, 1, 3, 3-tetramethylbutylphenol, Triton X-100 (TX-100), and anionic sodium dodecylsulfate (NaLS) surfactant micelles upon the spectrofluorimetric determination of pyrene is described. It was found that the intensity of the pyrene fluorescence is enhanced from 3 to 16 times in the presence of the micellar systems. Possible reasons for this micellar induced enhanced fluorescence are discussed. The spectral parameters, fluorescence lifetimes, quantum yields, lower detection limits, and analytical figures of merit for pyrene in CTAC, NaLS, TX-100, ethanol, and water are compared. The detection limit of pyrene in the presence of micelles (∼1.0 × 10−10M) is about an order of magnitude lower than that possible in alcohol alone. A brief discussion on the predicted general applicability of this new technique in fluorimetric analyses is also given.