Scanning Tunneling Microscopy ofE.coliRNA Polymerase Electrochemically Deposited onto an Au Substrate
作者:
Rebecca W. Keller,
Carlos Bustamante,
David G. Bear,
期刊:
AIP Conference Proceedings
(AIP Available online 1991)
卷期:
Volume 241,
issue 1
页码: 166-175
ISSN:0094-243X
年代: 1991
DOI:10.1063/1.41409
出版商: AIP
数据来源: AIP
摘要:
An electrodeposition method to bindE.coliRNA polymerase onto a monoatomically flat gold surface is described. The enzyme is imaged by the STM in a high humidity or water/glycerol environment which allows direct observation without a metal coating. The enzyme molecules appear as ordered arrays or disordered arrays depending on the deposition conditions used. Cyclic voltammetry reveals the blocking of the Au electrode as the electrodeposition proceeds and suggests that the attachment of the polymerase takes place by a physisorption mechanism and not by chemical bonding to the surface.
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