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Enhancement of Histological Detail Using Metanil Yellow as Counterstain in Periodic Acid Schiff's Hematoxylin Staining of Glycol Methacrylate Tissue Sections

 

作者: QuinteroIliana,   GrierHarry,   MuscatoMarilou,  

 

期刊: Biotechnic&Histochemistry  （Taylor Available online 1991）
卷期: Volume 66, issue 4  

页码: 169-172

 

ISSN:1052-0295

 

年代: 1991

 

DOI:10.3109/10520299109109964

 

出版商: Taylor&Francis

 

关键词: staining;glycol methacrylate;cytological detail;PAS-hematoxylin;metanil yellow

 

数据来源: Taylor

 

摘要:

Histological detail in sections from tissues embedded in glycol methacrylate was improved by counterstaining PAS/iron-hematoxylin stained sections with a dilute solution of metanil yellow. The addition of the counterstain increases contrast in tissue sections and highlights PAS-positive entities. The staining protocol provides sharp definition of tissue morphology, differentiates cell types and other tissue components and does not produce background staining.

 

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