Genetic Transformation of Carnation (Dianthus CaryophyllusL.)
作者:
期刊:
Biotechnology & Biotechnological Equipment
(Taylor Available online 1997)
卷期:
Volume 11,
issue 1-2
页码: 21-25
ISSN:1310-2818
年代: 1997
DOI:10.1080/13102818.1997.10818924
出版商: Taylor & Francis
数据来源: Taylor
摘要:
Transgenic plants of carnation (Dianthus caryophyllus) were obtained by Agrobacterium mediated transformation. A regeneration procedure was based on the leaf and petals as initial explants. Two different phytohormones- BAP and NAA were employed for the production of adventitious shoots. Transformation of the cultivars Lena, Line 84 and Yanita was performed with a plasmid (p2260 1 170) containig marker genes—npt II and hpt genes encoding neomycinephosphotransferase and hygromicinephosphotransferase, respectively. For cvs. Scania, Nasslada and Lena the plasmid vip 104-carring anti-sense CHS-A and nptII was used. Cultivar Regina was transformed with plasmid vip 165- GUS gene under chs-J promoter. Transformation efficiency varied among tested cvs. Presence of the npt II gene in plants was confirmed by PCR and Southern blot analysis. The expression of CHS-A was confirmed by Northern blot.
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