Effect of Human IGG1 Peptides on the Antigen-Specific Antibody Response of Mice in Vivo
作者:
KádárJ.,
SármayG.,
RajnavölgyiE.,
StanworthD.,
GergelyP.,
期刊:
Immunopharmacology and Immunotoxicology
(Taylor Available online 1992)
卷期:
Volume 14,
issue 3
页码: 611-623
ISSN:0892-3973
年代: 1992
DOI:10.3109/08923979209005413
出版商: Taylor&Francis
数据来源: Taylor
摘要:
AbstractThe effect of synthetic peptides - corresponding to the amino acid sequences 289-301 (Y48) and 293-301 (Y91) within the CH-2 domain in the human IgG1 was studied on the oxazolone-specific primary and secondary antibody response isotype distribution and on the sheep erythrocyte (SRBC)-specific primary IgM response. High responder (Ba1b/c) and low responder (C57B1/6) mice to oxazolone hapten were treated intraperitoneally with various doses of peptides simultaneously with the first and second contact sensitization. The relative levels of oxazolone-specific IgM, IgG3, IgG1, IgG2a and IgG2b antibodies were determined by a solid phase radioimmunoassay. Y48 and Y91 peptides in a dose range of 10−5-10−8M/animal enhanced the oxazolone-specific antibody response. This effect was more striking under suboptimal conditions: using smaller antigen dose for sensitization, cyclophosphamide pretreatment or using genetically low responder mice. SRBC-specific primary IgM response was enhanced by Y91 peptide, Y48 was ineffective.
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