A dynamic chiral stationary phase (CSP) prepared by hydrophobically bonding N-dodecyl diamide of (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid to octadecyl silica gel was successfully employed in resolving various racemic compounds containing a primary amino functional group. The racemic compounds resolved on the CSP include α-amino acids, α-amino esters, amino alcohols, amines, α-aminocarbonyl compounds, and fluoroquinolone antibacterial agents.