AbstractSex dependence of copper, zinc, iron nutritional status and hepatic lipid peroxidation was studied in gonadectomized, hormone substituted and sham‐operated male and female rats. The experiment was performed on H‐Wistar rats (106 ± 10 g) fed conventional laboratory food ad libitum for 12 weeks. The estrogen dependence of copper status is fully confirmed by the results of this study. In hormonally active females the malondialdehyde production was depressed which is supposed to be connected with high copper and low triglyceride levels in the liver. The anaemia observed in estrogen substituted rats may be a result of lower lipid peroxidation rate. The levels of retinol and alkaline phosphatase activity in sera are probably influenced by estrogen action. It is suggested that testosterone leads to acceleration of lipid peroxida