Use of Heparin-Sepharose for Detecting heparin-Binding growth Factor receptors in detergent Soluble Fractions
作者:
Alicia Torriglia,
PierreR. Blanquet,
期刊:
Analytical Letters
(Taylor Available online 1993)
卷期:
Volume 26,
issue 1
页码: 33-47
ISSN:0003-2719
年代: 1993
DOI:10.1080/00032719308016793
出版商: Taylor & Francis Group
关键词: cross-linking;heparin-Sepharose;solubilized FGF receptors;heparin-binding growth factors.
数据来源: Taylor
摘要:
Classical cross-linking technology has been found to be of very limited use in identifying solubilized receptors. A new assay based on the use of heparin-Sepharose was developed here for detecting receptors of fibroblast growth factors (FGFs) in detergent-soluble fractions. This new cross-linking assay is based on the heparin-binding properties of FGF. To this end, solubilized FGF receptors were cross-linked to radiolabelled acidic FGF on heparin-Sepharose. Standardization of the method was carried out by using: 1) crude CHAPS-soluble extracts from chinese hamster ovary cells which express human FGF receptor cDNA (FGFR-2); 2) CHAPS-soluble fractions purified by affinity chromatography from bovine retina. This approach might be a valuable tool for detecting receptors of all heparin-binding growth factors.
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