Alcian Blue Method for Demonstration ofHistoplasma duboisiiin Paraffin Sections
作者:
PereraP.A.C.,
期刊:
Journal of Histotechnology
(Taylor Available online 1989)
卷期:
Volume 12,
issue 3
页码: 220-222
ISSN:0147-8885
年代: 1989
DOI:10.1179/his.1989.12.3.220
出版商: Taylor&Francis
关键词: Alcian blue;African histoplasmosis;eosin;Histoplasma duboisii
数据来源: Taylor
摘要:
AbstractA special staining technique for demonstratingHistoplasma duboisiiin paraffin sections was developed during a study on the staining characteristics of this fungus, which acts as the causative organism for a type of fungal granuloma known as African histoplasmosis, found mostly in African countries. The method is relatively simple, reliable, and easily reproducible. It uses two common dyes, eosin and Alcian blue, that are readily available. The technique does not require any special fixatives and can be carried out on formalin fixed, parafin processed specimens. Because the method also eliminates the complicated and time-consuming special techniques previously used to demonstrate this fungus, it is more economical and time-saving. In paraffin sections,H. duboisiiare demonstrated as round to ovoid sky-blue circlets in rose-pink background. (J Histotechnol12:220, 1989).
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