AbstractA method for assessing the relative concentration and distribution of mineral in undecalcified bone sections is described. The procedure consists of gradually grinding plastic-embedded bone section to 100+M, exposing the sections to soft x-rays under vacuum on photographic film using an aluminum stepwedge as control. Maximum and minimum radiodensity in the photographic negative that correspond between the x-ray images of bone and the step-wedge were respectively compared. The total densitometric detection range of the system was arbitrarily divided into specified sub-ranges. The respective densitometric data for each sub-range was quantitated and expressed in numerical form.