Cytotoxic Assay of Circulating Thyroid Peroxidase Antibodies
作者:
WadeleuxP.,
WinandJ.,
RufJ.,
CarayonP.,
WinandR.,
期刊:
Autoimmunity
(Taylor Available online 1989)
卷期:
Volume 4,
issue 4
页码: 247-254
ISSN:0891-6934
年代: 1989
DOI:10.3109/08916938909014701
出版商: Taylor&Francis
关键词: Thyroid;anti-microsomal antibodies;cytotoxic antibodies;antithyroid peroxidase antibodies;thyroid peroxidase;microsomal antigen;autoimmunity
数据来源: Taylor
摘要:
This study describes an assay for the detection of cytotoxicity for thyroid cells in serum of patients with autoimmune thyroiditis. Quantative measurement may be performed by DNA or [3H] leucine incorporation determinations. The cytotoxic effect is localized in the gamma-globulin fraction, and is complement-mediated. It is thyroid specific i.e. it is not observed with fibroblasts and patients with other autoimmune diseases (patients with lupus erythematosis or glomerulonephritis) do not have cytotoxic antibodies directed against thyroid cells. The thyroid cytotoxicity is related to the presence of antimicrosomal antibodies and the effect of circulating antibodies is inhibited by human thyroid peroxidase. These results strengthen the possible implication of circulating antithyroid peroxidase antibodies in thyroid damage observed in autoimmune thyroiditis.
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