Isolation and Characterization of TGF-β2 and TGF-β5 from Medium Conditioned by Xenopus XTC Cells
作者:
RobertsAnita B.,
RosaFrédéric,
RocheNanette S.,
ColiganJohn E.,
GarfieldMark,
RebbertMartha L.,
KondaiahPaturu,
DanielpourDavid,
KehrlJohn H.,
WahlSharon M.,
DawidIgor B.,
SpornMichael B.,
期刊:
Growth Factors
(Taylor Available online 1990)
卷期:
Volume 2,
issue 2
页码: 135-147
ISSN:0897-7194
年代: 1990
DOI:10.3109/08977199009071500
出版商: Taylor&Francis
关键词: TGF-β;Xenopus laeins;mesoderm induction;HPLC
数据来源: Taylor
摘要:
AbstractTGF-β2 and -β5 have been purified from medium conditioned byXenopuscultured cells (XTC) and identified based on their N-terminal amino acid sequence analysis and biological activity. When applied in high concentrations,XenopusTGF-β2, like porcine TGF-β2, induces expression of mesodermal markers from culturedXenopusectodermal explants, whereas TGF-β5 is inactive in this assay. However, the TGF-β's could be separated from the major mesoderm-inducing activity present in XTC medium.XenopusTGF-β2 and -β5 are approximately equivalent to TGF-β1 in their abilities to inhibit the growth of mink lung CCL-64 cells, induce anchorage-independent growth of rat NRK cells, inhibit the proliferation and antibody secretion of human B-lymphocytes, and stimulate chemotaxis of human monocytes. These data establish the functional activity of TGF-β5 and suggest that more complex multicellular systems, in contrast to most isolated cells, discriminate between the different TGF-βs.
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