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Isolation and Characterization of TGF-β2 and TGF-β5 from Medium Conditioned by Xenopus XTC Cells

 

作者: RobertsAnita B.,   RosaFrédéric,   RocheNanette S.,   ColiganJohn E.,   GarfieldMark,   RebbertMartha L.,   KondaiahPaturu,   DanielpourDavid,   KehrlJohn H.,   WahlSharon M.,   DawidIgor B.,   SpornMichael B.,  

 

期刊: Growth Factors  (Taylor Available online 1990)
卷期: Volume 2, issue 2  

页码: 135-147

 

ISSN:0897-7194

 

年代: 1990

 

DOI:10.3109/08977199009071500

 

出版商: Taylor&Francis

 

关键词: TGF-β;Xenopus laeins;mesoderm induction;HPLC

 

数据来源: Taylor

 

摘要:

AbstractTGF-β2 and -β5 have been purified from medium conditioned byXenopuscultured cells (XTC) and identified based on their N-terminal amino acid sequence analysis and biological activity. When applied in high concentrations,XenopusTGF-β2, like porcine TGF-β2, induces expression of mesodermal markers from culturedXenopusectodermal explants, whereas TGF-β5 is inactive in this assay. However, the TGF-β's could be separated from the major mesoderm-inducing activity present in XTC medium.XenopusTGF-β2 and -β5 are approximately equivalent to TGF-β1 in their abilities to inhibit the growth of mink lung CCL-64 cells, induce anchorage-independent growth of rat NRK cells, inhibit the proliferation and antibody secretion of human B-lymphocytes, and stimulate chemotaxis of human monocytes. These data establish the functional activity of TGF-β5 and suggest that more complex multicellular systems, in contrast to most isolated cells, discriminate between the different TGF-βs.

 

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