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A New Method for Melanin at Acid pH

 

作者: GarveyWinsome,   FathiArleen,   BigelowFrancine,   JimenezCarmencita,   CarpenterBlair,  

 

期刊: Journal of Histotechnology  （Taylor Available online 1990）
卷期: Volume 13, issue 4  

页码: 279-282

 

ISSN:0147-8885

 

年代: 1990

 

DOI:10.1179/his.1990.13.4.279

 

出版商: Taylor&Francis

 

关键词: bleaching;melanin;pigments;silver impregnation

 

数据来源: Taylor

 

摘要:

AbstractA simple, reliable, consistent and economical nonbuffered acid acid method for melanin is presented. After a short presensitization in silver, the sections are treated in gum mastic and then immersed in a physical developer consisting of hydroquinone, gum mastic, and a very low concentration of silver nitrate. The sections are placed in thiosulfate and counterstained with nuclear fast red. The entire procedure takes under 40 minutes to perform. (The J Histotechnology13:279, 1990)

 

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