Étude comparative de la transformation et de la fusion induite par les polyéthyléneglycols chezAcholeplasma laidlawiiB
作者:
J. L. Goulay,
J. C. Darbord,
A. Desvignes,
期刊:
Canadian Journal of Microbiology
(NRC Available online 1984)
卷期:
Volume 30,
issue 1
页码: 40-44
ISSN:0008-4166
年代: 1984
DOI:10.1139/m84-007
出版商: NRC Research Press
数据来源: NRC
摘要:
This study showed inAcholeplasma laidlawiithe possibility of transformation and fusion of protoplasts after induction by polyethylene glycol. Recombination in a genome of the resistance to chloramphenicol and neomycin has been obtained. Minimal inhibitory concentraiton of these antibiotics towards the recombined strain are identical to those measured with the parental strains. The most suitable experimental conditions have been studied. It appeared that the fusion, which is carried out more easily than the transformation, led to a higher frequency of recombination.[Translated by the journal]
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