Liquid Chromatography Determination of Thiopentone in Human Plasma
作者:
C. Salvadori,
R. Farinotti,
Ph. Duvaldestin,
A. Dauphin,
期刊:
Therapeutic Drug Monitoring
(OVID Available online 1981)
卷期:
Volume 3,
issue 2
页码: 171-176
ISSN:0163-4356
年代: 1981
出版商: OVID
关键词: Liquid chromatography;Thiopentone;Plasma concentrations;Carbamazepine
数据来源: OVID
摘要:
A rapid microprocedure for the liquid chromatographic analysis of thiopentone in plasma is described. Reversed-phase liquid chromatography was performed on a microparticulate C18column using as a mixture of 60% methanol/40% water as mobile phase and ultraviolet detection (290 nm). The method used carbamazepine as internal standard and ethyl acetate as extraction solvent. The analytical recovery was 95%; the reproducibility, 6.5%; and the sensitivity limit of detection, 0.2 mg/liter. The method has been used in preliminary studies to determine the plasma concentrations in patients receiving thiopentone by the intravenous route. A method using direct injection is described.
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