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Electron immunoperoxidase staining in sections of unfixed pure cultures ofStreptococcus mutans

 

作者: BertholdPeter,   H.C.,   E.C.,  

 

期刊: Acta Odontologica Scandinavica  （Taylor Available online 1982）
卷期: Volume 40, issue 5  

页码: 283-287

 

ISSN:0001-6357

 

年代: 1982

 

DOI:10.3109/00016358209024071

 

出版商: Taylor&Francis

 

关键词: Electron microscopy;fixation;immunohistochemistry

 

数据来源: Taylor

 

摘要:

Unfixed as well as glutaraldehyde-fixed pelleted cells from pure cultures ofStreptococcus mutans subspecies sobrinus(B13) were embedded in glycolme-thacrylate. Uitrathin were sections immunoperoxidase labelled with rabbit anti-S.mutans sobrinusγ-globulin. It was found that unfixed specimens became labelled as precisely and as distinctly as did the fixed specimens. All controls were negative.

 

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