A specific subtype C of human immunodeficiency virus type 1 circulates in Brazil
作者:
Marcelo Soares,
Tulio de Oliveira,
Rodrigo Brindeiro,
Ricardo Diaz,
Ester Sabino,
Luís Brigido,
Ivone Pires,
Mariza Morgado,
Maria Dantas,
Draurio Barreira,
Paulo Teixeira,
Sharon Cassol,
Amilcar Tanuri,
期刊:
AIDS
(OVID Available online 2003)
卷期:
Volume 17,
issue 1
页码: 11-21
ISSN:0269-9370
年代: 2003
出版商: OVID
关键词: subtype C;drug resistance mutations;subtype polymorphism;HIV in primary infection;signature sequence
数据来源: OVID
摘要:
Objective:To characterize the subtype C strains of HIV type 1 that circulate in Brazil, especially those originated from the southern part of the country.Design and methods:One hundred and twelve HIV-1-positive subjects had their plasma viral RNA extracted. Protease (PR) and reverse transcriptase (RT) genomic regions were polymerase chain reaction-amplified and sequenced for subtype determination. Subtype C strains were selected and compared to other strains of this subtype from the database, and specific amino acid signature patterns were searched.Results:Brazilian subtype C viruses form a very strong monophyletic group when compared to subtype C viruses from other countries and presented specific signature amino acids. Recombinants between subtype C and B viruses have been documented in areas of co-circulation. The incidence of primary PR and RT inhibitor resistance mutations in drug-naïve subjects was observed. An increasing number of secondary resistance mutations was also seen, some of which are characteristic of subtype C-related sequences.Conclusions:Introduction of subtype C of HIV-1 in Brazil was likely a single event of one or a mixture of similarly related strains. Recombination between subtype C and B viruses is an ongoing process in the country. Primary and secondary drug resistance mutations were observed, although some of the secondary mutations could be associated with subtype C molecular signatures. Subtype-specific polymorphisms of PR and RT sequences found in this subtype C Brazilian variant might influence this emergence and have an impact on HIV treatment and on vaccine development in the country.
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