Interaction of Heparin-Binding EGF-Like Growth Factor (HB-EGF) with the Epidermal Growth Factor Receptor: Modulation by Heparin, Heparinase, or Synthetic Heparin-Binding HB-EGF Fragments
作者:
BesnerGail E.,
WheltonDiane,
CrissmanMelissa A.,
SteffenChristy L.,
KimGregory Y.,
BrigstockDavid R.,
期刊:
Growth Factors
(Taylor Available online 1992)
卷期:
Volume 7,
issue 4
页码: 289-296
ISSN:0897-7194
年代: 1992
DOI:10.3109/08977199209046411
出版商: Taylor&Francis
关键词: heparin;HB-EGF;EGF;peptide mapping;extracellular matrix
数据来源: Taylor
摘要:
AbstractThe binding of heparin-binding EGF-like growth factor (HB-EGF) to the epidermal growth factor (EGF) receptor of human endometrial carcinoma cells was compared to that of EGF using an125I-EGF radioreceptor assay. The inhibitory effect of HB-EGF on125I-EGF binding was reversed either in the presence of heparin (but not by chondroitin sulfate) or by pre-treating the cells with heparinase. These treatments did not affect the binding of EGF to its receptor. To map potential regions in the HB-EGF molecule that mediate its heparin-dependent interaction with the EGF receptor, HB-EGF peptides were synthesized that were non-homologous to EGF. Accordingly residues 20-25 and 36-41, but not residues 8-19, of HB-EGF were found to be (i) heparin-binding and (ii) modulators of HB-EGF (but not of EGF) binding to the EGF receptor.
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