PURIFICATION OF PEG-PROTEIN CONJUGATES BY COUNTERCURRENT DISTRIBUTION IN AQUEOUS TWO-PHASE SYSTEMS
作者:
Terasut Sookkumnerd,
JamesT. Hsu,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 2000)
卷期:
Volume 23,
issue 4
页码: 497-503
ISSN:1082-6076
年代: 2000
DOI:10.1081/JLC-100101467
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Purification of PEG-protein conjugates by countercurrent distribution (CCD) in aqueous two-phase systems is reported. By using CCD in 19.36% PEG 3400/14.68% potassium phosphate, we can separate PEG-lysozyme conjugates, which contains an average number of 1.14 PEG per lysozyme, into three peaks with 55 number of transfers. Further analysis of the samples reveals that these three peaks mainly represent unmodified lysozyme, (PEG)1-lysozyme and (PEG)2-lysozyme.
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