Embryonic limb bud organ culture in assessment of teratogenicity of environmental agents
作者:
D. M. Kochhar,
期刊:
Teratogenesis, Carcinogenesis, and Mutagenesis
(WILEY Available online 1982)
卷期:
Volume 2,
issue 3‐4
页码: 303-312
ISSN:0270-3211
年代: 1982
DOI:10.1002/1520-6866(1990)2:3/4<303::AID-TCM1770020311>3.0.CO;2-I
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: screening system;mouse embryo;organogenesis;cartilage
数据来源: WILEY
摘要:
AbstractThe limb bud organ culture system offers a variety of endpoints which may be monitored in the screening process. These are: cell proliferation, differential growth, morphogenetic cell death, size and shape of limb parts, chondrogenesis, collagen or proteoglycan biosynthesis, etc. Essentials of the system including various parameters of normal limb bud development in vitro are described. These parameters serve to gauge the effects of test chemicals with unknown hazard potential. Validation has been carried out only to a limited extent. Further, it needs to be combined with an efficient drug metabolizing preparation before it can achieve its full potential as a short‐term screening syste
点击下载:
PDF
(933KB)
返 回