Amperometric Immunosensor for the Detection of 2,4-Dichlorophenoxyacetic Acid (2,4-D) in Water
作者:
M. Wilmer,
D. Trau,
R. Renneberg,
F. Spener,
期刊:
Analytical Letters
(Taylor Available online 1997)
卷期:
Volume 30,
issue 3
页码: 515-525
ISSN:0003-2719
年代: 1997
DOI:10.1080/00032719708001798
出版商: Taylor & Francis Group
关键词: Sequential injection analysis;immunosensor;amperometric;phenoxycarbonic acids;2,4-D;monoclonal antibodies;drinking water
数据来源: Taylor
摘要:
An amperometric immunosensor for the determination of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) in water has been developed using sequential injection analysis techniques. The system is based on a rapid competitive enzyme immunoassay employing an alkaline phosphatase-labeled monoclonal antibody directed against the herbicide and an immunoreactor with 2,4-D immobilized via bovine serum albumin either to Eupergit in a column or directly to the surface of a glass capillary. The detection limit of the immunosensor at 0.1 μg 2,4-D/l without enrichment of the analyte makes automatic measurements of 2,4-D in drinking and ground water feasible.
点击下载:
PDF (341KB)
返 回