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Microdosage Rapide et Sensible de Proteines Immobilisees sur Support Insoluble: Applications aux Anticorps Immobilises sur Polystyrene

 

作者: R. Couturier,   B. Perrin,   G. Favre-bonvin,   A. Ville,  

 

期刊: Analytical Letters  （Taylor Available online 1985）
卷期: Volume 18, issue 17  

页码: 2209-2218

 

ISSN:0003-2719

 

年代: 1985

 

DOI:10.1080/00032718508067982

 

出版商: Taylor & Francis Group

 

关键词: polystyrène;anticorps;bleu de coomassie G-250;spectrophotométrique

 

数据来源: Taylor

 

摘要:

A rapid and sensitive method for the quantitation of microgram antibodies immobilized on polystyrene is proposed. A procedure of protein staining on polystyrene with coomassie, brilliant blue G 250 follows Beer's law after release of the coloured complex. The lower limits of detection are determined.

 

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