A Technique for Embedding Undecalcified Bone Samples for Detecting Alpha-Emitters Using Vacuum Impregnation with Spurr's Resin
作者:
HainesJ. W.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 1
页码: 45-49
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110005
出版商: Taylor&Francis
关键词: undecalcified bone;Spurr's resin;CR-39;alpha-emitters
数据来源: Taylor
摘要:
A method has been developed by which large samples of mineralized bone, containing an alpha-emitter, can be embedded in Spurr's resin in a fraction of the time required by conventional methods. Bone samples were freeze-dried or fixed and dried prior to impregnation with Spurr's resin under vacuum. Sections were cut for the preparation of either alpha-track or fission-track autoradiographs using the solid state detector CR-39. This method is applicable to samples containing a mobile form of a radionuclide that may be translocated during the processes of fixation and dehydration of the specimen.
点击下载:
PDF (1092KB)
返 回