Expression of PEP‐19 inhibits apoptosis in PC12 cells
作者:
Joseph Erhardt,
Jeffrey Legos,
Roy Johanson,
J Slemmon,
Xinkang Wang,
期刊:
NeuroReport
(OVID Available online 2000)
卷期:
Volume 11,
issue 17
页码: 3719-3723
ISSN:0959-4965
年代: 2000
出版商: OVID
关键词: Apoptosis;Calmodulin;PEP-19;PC12
数据来源: OVID
摘要:
PEP-19 is a calmodulin-regulatory protein found specifically within neurons, though cellular functions of this protein have not been determined. In an effort to define potential effects of PEP-19, PC12 cell lines expressing this protein were generated and subjected to apoptotic stimuli. As measured by LDH release, cell death in PEP-19 expressing cells was 2-to 5-fold less following u.v. irradiation, and 2- to 4-fold less following staurosporine treatment than controls. Additionally, PEP-19-expressing cells displayed decreased DNA ladder formation, chromatin condensation, and caspase activation following staurosporine treatment. Overall, these results demonstrate that PEP-19 can inhibit apoptotic processes in PC12 cells, suggesting a potential regulatory mechanism for pathways leading to cell death.
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