Immunomagnetic isolation of human developing motor neurons
作者:
Vincenzo Silani,
Andrea Brioschi,
Massimiliano Braga,
Andrea Ciammola,
Feng Zhou,
Christiana Bonifati,
Antonia Ratti,
Antonio Pizzuti,
Mauro Buscaglia,
Guglielmo Scarlato,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 6
页码: 1143-1147
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: ChAT;Isolation;Motor neuron;p75-NGFR;Spinal cord
数据来源: OVID
摘要:
HUMAN motor neuron (MN) isolation provides a critical tool to study neurophysiological properties and the effects of molecules of clinical relevance on isolated neurons. We developed an immunomagnetic separation technique based on specific MN antigen recognition for nerve growth factor receptor (p75-NGFR). We cultured an average of 250 000 cells from the anterior horns of a single cord (four specimens at postconception Weeks 6.0, 7.2, 8.0, and 8.3). At day 7in vitro(DIV), choline acetyltransferase (ChAT) and/or p75-NGFR-expressing cells (MNs) represented 72 ± 2% of the total growing cells. MNs survived for at least 4 weeks in biochemically defined medium. The immunomagnetic separation method has been demonstrated to be effective, reproducible, and quantitative for separation of MNs.
点击下载:
PDF
(658KB)
返 回