Are cardiac G-proteins altered in rat models of hypertension?
作者:
Martin Michel,
Otto-Erich Brodde,
Paul Insel,
期刊:
Journal of Hypertension
(OVID Available online 1993)
卷期:
Volume 11,
issue 4
页码: 355-363
ISSN:0263-6352
年代: 1993
出版商: OVID
关键词: Spontaneously hypertensive rat;renal hypertension;G-protein;heart
数据来源: OVID
摘要:
Objective:To determine whether alterations in cardiac G-protein number or function, or both, are involved in the desensitization of adenylate cyclase responsiveness in the hypertensive stateDesign:Quantitation of G-protein subunits in myocardial membranes from four different rat models of hypertension in comparison with respective normotensive ratsMethods:We compared male and female adult spontaneously hypertensive rats (SHR) with age- and sex-matched Wistar-Kyoto (WKY) rats, rats from the highest-pressure quartile from an F2generation of WKY x SHR hybrids with those from the lowest-pressure quartile, and one-kidney, one clip renal hypertensive with shamoperated rats. The function of GSαwas quantitated by reconstitution of cardiac cholate extracts into cyc—cell membranes with subsequent measurement of NaF-stimulated adenylate cyclase activity. The amounts of immunodetectable CSα, Giα,Gqα, and Gβ were determined from quantitative Western blotting experiments with[125l]-protein A detectionResults:None of the parameters investigated differed significantly between hypertensive and normotensive rats in any of the models investigatedConclusion:We conclude that major quantitative alterations in cardiac Gs, Gjor Gqare not a general feature of the hypertensive state
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