Effect of different treatments on the conversion of ‘Hayward’ kiwifruit synthetic seeds to whole plants following encapsulation ofin vitro‐derived buds
作者:
M. Adriani,
E. Piccioni,
A. Standardi,
期刊:
New Zealand Journal of Crop and Horticultural Science
(Taylor Available online 2000)
卷期:
Volume 28,
issue 1
页码: 59-67
ISSN:0114-0671
年代: 2000
DOI:10.1080/01140671.2000.9514123
出版商: Taylor & Francis Group
关键词: kiwifruit;Actinidia deliciosa;artificial seed;encapsulation;micropropagation;synseed;sodium‐calcium alginate
数据来源: Taylor
摘要:
Encapsulated buds excised fromin vitroproliferated shoots of the ‘Hayward’ kiwifruit (Actinidia deliciosa(A. Chev.) C. F. Liang et A. R. Ferguson) can be used for non‐embryogenic synthetic seed production. Three experiments were carried out to evaluate the aptitude of apical and axillary buds (microcuttings) towards encapsulation and synthetic seed productionJuland to find the treatments able to induce conversion of the synthetic seeds to whole plantlets. ‘Hayward’ proliferating shoots are useful sources of microcuttings for encapsulation and synseed production, since a proliferation protocol is already available. Encapsulation, although considered necessary, depressed microcuttings’ vigour and vegetative activity. Cold treatments provided to thein vitroproliferating mother shoots boosted bud vigour and subsequent conversion. Increase of concentration of sucrose in some steps of the protocol also enhanced conversion, which in some conditions reached a rate of 57.5%. Potential applications of encapsulation and the synthetic seed technology in kiwifruit germplasm exchange and commerce are also discussed.
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