Identification of two cell surface proteins of rat brain oligodrocytes in culture
作者:
F. M. Pari,
A. Espinosa de los Monteros,
G. Roussel,
J. L. Nussbaum,
期刊:
Journal of Neuroscience Research
(WILEY Available online 1987)
卷期:
Volume 17,
issue 1
页码: 60-64
ISSN:0360-4012
年代: 1987
DOI:10.1002/jnr.490170109
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: cell surface marker;culture‐conditioned medium;Wolfgram proteins;immunoblotting
数据来源: WILEY
摘要:
AbstractAntibodies specific for the surface of oligodendrocytes were prepared by incubating living cultures of pure oligodendrocytes with a crude anti‐oligodendrocyte antiserum. These specific antibodies, when used in the technique of immunoelectroblotting, led to the characterization of at least two major plasma membrane proteins of 43 kilodaltons (kDa) and 53 kDa, respectively, as accessible at the external surface of the oligodendrocytes. The 53‐kDa protein was also found in oligodendrocyte‐conditioned medium in significant amounts. Additional oligodendrcyte surface proteins were also detected in the Wolfgram protein fra
点击下载:
PDF
(579KB)
返 回