The wheat proteins puroindoline‐a and α1‐purothionin induce nodal swelling in myelinated axons
作者:
César Mattei,
Khalil Elmorjani,
Jordi Molgó,
Didier Marion,
Evelyne Benoit,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 17
页码: 3803-3803
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Confocal laser scanning microscopy;Fluorescent staining;FM1-43;Myelinated axons;Node of Ranvier;Puroindoline-a;α1-Purothionin;Swelling;Volume;Wheat proteins
数据来源: OVID
摘要:
The effects of two basic cysteine-rich lipid-binding proteins isolated from wheat seedlings, puroindoline-a and α1-purothionin, were studied on single frog myelinated axons stained with the fluorescent dye FM1-43 using confocal laser scanning microscopy. During exposure to either puroindoline-a or α1-purothionin (10 and 100 μM) a marked swelling of nodes of Ranvier was observed, provided NaCl was present in the external solution. It is suggested that these proteins increase the internal osmolality by forming pores in the axonal membrane and induce water influx to compensate for such an increase. Moreover, in the presence of α1-purothionin (100 μM), the intensity of the axonal staining with FM1-43 was increased. It is the first time, to our knowledge, that basic proteins containing domains of a cysteine-rich repeated motif are reported to produce swelling and water movements across neuronal cell membranes.
点击下载:
PDF
(1947KB)
返 回