Frequency of dual infections of corneas with HIV-1 and HHV-6
作者:
QaviHamida Q.,
GreenMary T.,
SeGallGary K.,
LewisDorothy E.,
HollingerF. Blaine,
期刊:
Current Eye Research
(Taylor Available online 1992)
卷期:
Volume 11,
issue 4
页码: 315-323
ISSN:0271-3683
年代: 1992
DOI:10.3109/02713689209001785
出版商: Taylor&Francis
数据来源: Taylor
摘要:
In the current study, 35 pairs of corneas from asymptomatic carriers of HIV-1 and ten pairs from AIDS patients were analyzed for the presence of HIV-1 and HHV-6. The tissues were evaluated for viral antigens, transcripts, DNA sequences and intact and infectious virus. Three corneas from two asymptomatic carriers of HIV-1 and three corneas from two AIDS patients were culture positive for HIV-1. One of the three HIV-1 positive corneas from an asymptomatic HIV-1 carrier also was culture positive for HHV-6. Two of the tissue culture positive corneas from asymptomatic HIV-1 carriers and two from AIDS patients also tested positive for HIV-1 transcriptional activity byin situhybridization. The label denoting the transcriptional activity was limited to stromal keratocytes. Most significantly, we were able to demonstrate the presence of HIV-1 particle(s) in sections and cultured PBMC from one of the HIV-1 culture positive corneas. PBMC from the same cornea also contained herpes virus particles. This report strengthens our earlier findings that HIV-1 and HHV-6 can invade corneal tissue, which emphasizes the importance of vigorous screening of corneal donors, specifically donors with HIV-1 exposure.
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