ABSTRACTNucleotide sequences from a highly variable region of large subunit (25–28S) rRNA (D2 region, ca. 220 nucleotides) were determined for seven strains ofG. pulicarisand also for two strains each of threeG. fujikuroivarieties in order to assess the amount of within species or within variety sequence variation and to determine whether sequences of the varieties would be different in this rRNA region.One variable nucleotide position was found among theG. pulicarisstrains; all other readable bases were the same. No sequence differences were found among strains of eachG. fujikuroivariety. TwoG. fujikuroivarieties (intermediumandfujikuroi) had the same sequence and the third variety(moniliformis)differed from them at two nucleotide positions.