Relationship between Cytosolic Ca2+Level and Contractile Tension in Canine Basilar Artery of Chronic Vasospasm
作者:
Takeshi Yamada,
Yu-ichi Tanaka,
Kiyoshige Fujimoto,
Noboru Nakahara,
Soji Shinoda,
Toshio Masuzawa,
期刊:
Neurosurgery
(OVID Available online 1994)
卷期:
Volume 34,
issue 3
页码: 496-504
ISSN:0148-396X
年代: 1994
出版商: OVID
关键词: Basilar artery;Calcium;Dog;Smooth muscle;Vasospasm
数据来源: OVID
摘要:
IN ORDER TO study the role of the Ca2+/calmodulin/myosin light chain kinase system in the development of chronic vasospasm caused by subarachnoid hemorrhage, the cytosolic Ca2+level ([Ca2+]i), measured with fura-2, a fluorescent Ca2+indicator, and contractile tension were measured simultaneously, and their quantitive correlation was examined in basilar arterial tissue obtained from the canine “two-hemorrhage” model. Sixteen adult mongrel dogs were divided into two groups, control (n = 8) and vasospasm (n = 8), and were killed 7 days after the first experimental subarachnoid hemorrhage. In basilar arterial tissue loaded with fura-2, 1) [Ca2+]iin the resting condition was not significantly different between the two groups; 2) the increment in [Ca2+]iinduced by 40 mmol/L K+stimulation was significantly smaller in vasospastic tissue (P< 0.01); and 3) 40 mmol/L K+-induced tension development per cross-sectional area for a fixed increment in [Ca2+]iwas significantly greater in vasospastic tissue (P< 0.01). In tissue not loaded with fura-2, active myogenic tone, which was tentatively represented by the ratio of tonus relaxed with 10−4mol/L papaverine to initial resting tone, was significantly greater in vasospastic tissue (P< 0.05). These findings, coupled with recent reports concerning the ratio of phosphorylated myosin light chain, indicate the following about vasospastic arterial tissue in vitro: 1) in the resting condition, augmented myogenic tone, which is not accompanied by [Ca2+]ielevation, is probably not attributable to the Ca2+/calmodulin/myosin light chain kinase system, and by contrast, 2) at a higher level of [Ca2+]i, a stronger contraction for a fixed increment in [Ca2+]iis induced by Ca2+sensitization mechanisms of the calmodulin/myosin light chain kinase system.
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