AbstractGenetics, urinary chemosignals and related social influences, and ambient conditions affect reproduction in female mice. Five experiments tested the effects of environmental stressors on age at first vaginal estrus in female house mice. Environmental disruption in the form of changing the cage bedding and/or nesting material at various prescribed intervals resulted in different degrees of puberty delay relative to non‐disrupted control mice. Disruption in the form of a male chasing the female for one to three 15‐min intervals each day or the female being trapped and held in a live‐trap for one to three 15‐min intervals each day resulted in delays in puberty for treatments involving multiple daily disruptions. Food deprivation, but not water deprivation, influenced the onset of puberty. Variations in temperature and humidity resulted in differences in the age of puberty; low but not high temperatures and extremely low humidity levels delayed sexual mat