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Incorporation of14C stearic acid in lens organ culture

 

作者: AlbersBarbara,   BunchFrank T.,  

 

期刊: Current Eye Research  (Taylor Available online 1982)
卷期: Volume 2, issue 4  

页码: 233-237

 

ISSN:0271-3683

 

年代: 1982

 

DOI:10.3109/02713688209011624

 

出版商: Taylor&Francis

 

数据来源: Taylor

 

摘要:

14C stearic acid was incubated in organ culture of rabbit lens for 6, 12 and 20 hours. Stearic acid did penetrate the lens and was incorporated into triacyl-glycerides and phospholipids. It also served as a carbon source for cholesterol synthesis. Individual fatty acids were isolated and radioactivity measurements were made. Incorporation of label was found in C12:0, C14:0 and C16:0 fatty-acids, indicatingde novosynthesis in the lens; incorporation was found in C16:1 and C18:1, indicatingδ9 desaturase activity, and incorporation was found in the fatty acids of 20 carbons or longer, indicating the presence of a fatty acid elongation system in the lens.

 

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