A Computer‐based image analysis system for biocide screening
作者:
B Wigglesworth‐Cooksey,
K E Cooksey,
期刊:
Biofouling
(Taylor Available online 1996)
卷期:
Volume 10,
issue 1-3
页码: 225-237
ISSN:0892-7014
年代: 1996
DOI:10.1080/08927019609386282
出版商: Taylor & Francis Group
关键词: Amphora;diatom;image‐analysis;environmental toxicology;antifoulant;calcium
数据来源: Taylor
摘要:
A computer‐based image analysis system that measures the behavioral response of the marine diatomAmphora coffeaeformisto potentially toxic challenges is described. At any one time the system is capable of measuring in a population of about 80 cells, changes in the number motile, their speed, direction, and if they are turning, their angular velocity and acceleration. Chemical compounds that interfere with the generation of energy, protein or glycoprotein synthesis, calcium homeostasis and cytoskeletal activity, change the motile behavior of the cell. A pre‐requisite of motility is that the cells be attached to a substratum. It is proposed that the system, which requires only a 1–2 minute data collection period per replicate, could be used to screen for potential antifouling activity in chemical agents, and it is speculated that it could be used also for measuring the physicochemical interaction between attached motile organisms and their substratum, as well as in environment toxicology measurements.
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