The Fibrinolytic Activity of Staphylokinase Mutants in the Fibrin Plate Assay
作者:
J. Hauptmann,
K.-H. Gührs,
M. Hartmann,
B. Schlott,
期刊:
Pathophysiology of Haemostasis and Thrombosis
(Karger Available online 1995)
卷期:
Volume 25,
issue 6
页码: 272-276
ISSN:1424-8832
年代: 1995
DOI:10.1159/000217172
出版商: S. Karger AG
关键词: Fibrin plate;Staphylokinase;Streptokinase;Tissue-type plasminogen activator
数据来源: Karger
摘要:
The fibrinolytic activity of the new plasminogen activator, recombinant staphylokinase, was compared to that of streptokinase and tissue-type plasminogen activator in the fibrin plate assay. The pattern of fibrinolysis by staphylokinase on fibrin plates differs from the other plasminogen activators. A number of mutants of staphylokinase with various amino acids in position 26 substituted for methionine in wild-type staphylokinase were compared with respect to their fibrinolytic potencies. Only the mutants with cysteine or leucine in this position have a fibrinolytic activity comparable to wild-type staphylokinase. The results on the fibrinolytic activities in the fibrin plate assay correlate with those of a plasmin generation assay, the latter is, however, less sensitive.
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