Spectrofluorometric Determination of Serum Lithium Using 1, 8-Dihydroxyanthraquinone
作者:
Kim Wheeling,
GaryD. Christian,
期刊:
Analytical Letters
(Taylor Available online 1984)
卷期:
Volume 17,
issue 3
页码: 217-228
ISSN:0003-2719
年代: 1984
DOI:10.1080/00032718408065280
出版商: Taylor & Francis Group
关键词: manic depressive;lithium;serum;protein-free-filtrate;spectrofluorometry
数据来源: Taylor
摘要:
Serum samples are deproteinized with an equal volume of acetone and 20 μl of the filtrate is added to 1 ml of 2.5 × 10−4MKOH and 1.0 × 10−4M 1, 8-dihydroxyanthraquinone in 90% (V/V) acetone. Single point calibration with spiked serum at 1.5 mmol/l is used with unspiked serum as blank. Fluorescence emission is measured at 618 nm, with excitation at 410 nm. Samples analyzed by the described method and by atomic absorption spectrometry showed a correlation coefficient of 0.985. The relative responses for lithium in water, serum eletrolyte matrix and serum were 100, 57, and 44, respectively. The Li response in serum relative to that for Na, K, Ca, and Mg was 480, 600, 33, and 27, respectvely.
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