Simultaneuous Determination of Enalpril Maleate and Hydrochlorothiazide in Tablets by Reversed Phase HPLC
作者:
P.B. Shetkar,
V.M. Shinde,
期刊:
Analytical Letters
(Taylor Available online 1997)
卷期:
Volume 30,
issue 6
页码: 1143-1152
ISSN:0003-2719
年代: 1997
DOI:10.1080/00032719708004044
出版商: Taylor & Francis Group
关键词: Enalpril Maleate;Hydrochlorothiazide p-hydroxy phenyl acetamide;RP - HPLC
数据来源: Taylor
摘要:
A new simple, precise, rapid and selective RP-HPLC method has been developed for the simultaneous determination of Enalpril maleate and Hydrochlorothiazide from formulations, using 0.025M orthophosphoric acid [(adjusted to pH 3.0 with triethyl amine (TEA)]: Acetonitrile (84 16 V/V), as a mobile phase at a flow rate of 1.0 ml/min and μ Bondapak C18column (10 micron, 30cm × 3.9 mm) as a stationary phase. Detection was carried out using a UV detector at 226 nm. p-hydroxy phenyl acetamide (PHPA) was used as an internal standard. Linearity range and percentage recoveries for Enalpril maleate and Hydrochlorothiazide were 40–200 μg/ml and 50–500 μg/ml, 99.74 and 99.69 respectively. Minimum detection limit obtained was 0.8 μg/ml and 0.4 μg/ml for Enalpril maleate and Hydrochlorothiazide respectively.
点击下载:
PDF (291KB)
返 回