Reconstruction from serial sections: A tool for developmental biology. Application to Hox genes expression in chicken wing buds
作者:
J‐C Olivo,
J‐C Izpisúa‐Belmonte,
C Tickle,
C Boulin,
D Duboule,
期刊:
Bioimaging
(WILEY Available online 1993)
卷期:
Volume 1,
issue 3
页码: 151-158
ISSN:0966-9051
年代: 1993
DOI:10.1002/1361-6374(199309)1:3<151::AID-BIO3>3.0.CO;2-N
出版商: IOP Publishing Ltd
关键词: serial sections;registration;3‐D reconstruction;developmental biology;Hox genes
数据来源: WILEY
摘要:
AbstractA method for analysing the localization of the Hox‐4 gene complex by three‐dimensional reconstruction of serial cross‐sections is presented. It allows the simultaneous visualization of three Hox expression domains on the same reconstructed embryo and the study of the effect of a graft of chicken polarizing region on thede novoexpression of the genes of the HOX‐4 complex. The method consists of registering the sections, segmenting the biological patterns and finally displaying the reconstructed embryo. The precise relationships between the transcript domains of the three genes can thus be assessed in either the normal wing bud or upon pattern modif
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