Nuclear Staining ofColletotrichum GloeosporioidesF. SP.MalvaeConidia with Fluorescent and Nonfluorescent Stains
作者:
GantottiB. V.,
WoodskeD. V. G.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1991)
卷期:
Volume 66,
issue 1
页码: 7-13
ISSN:1052-0295
年代: 1991
DOI:10.3109/10520299109110541
出版商: Taylor&Francis
数据来源: Taylor
摘要:
Six different staining techniques were evaluated for their suitability to stain nuclei ofColletotrichum gloeosporioidesf. sp.malvae(C.g.m.) spores. Of the three fluorescent stains, DAPI (4',6-diamidino-2-phenylindole) and bisbenzimide (Hoechst 33258) stained spore nuclei well; mithramycin did not. To achieve consistent results with the bisbenzimide staining protocol, the spores had to be fixed prior to staining and the stain had to be supplemented with Triton X-100. Both safranin O and Giemsa were suitable nonfluorescent staining techniques; lomofungin was not. Safranin O staining was simple and rapid. However, reproducibility was better if the spore suspension and KOH droplets were rapidly mixed prior to adding the stain. There was no significant difference in the percentages of uninucleate and binucleate spores observed in spore preparations stained with DAPI, bisbenzimide, safranin O or Giemsa. Bisbenzimide and safranin O were found to be simple, rapid and reliable fluorescent and nonfluorescent techniques, respectively, for staining nuclei of C.g.m. spores.
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