CR1 Density Polymorphism and Expression on Erythrocytes of Patients with Systemic Lupus Erythematosus
作者:
KissE.,
CsipöI.,
CohenJ. H. M.,
ReveilB.,
KåvaiM.,
SzegediGy.,
期刊:
Autoimmunity
(Taylor Available online 1996)
卷期:
Volume 25,
issue 1
页码: 53-58
ISSN:0891-6934
年代: 1996
DOI:10.3109/08916939608994726
出版商: Taylor&Francis
关键词: Systemic lupus erythematosus CR1 erythrocytes gene amplificationHindIIIdigestion
数据来源: Taylor
摘要:
The present study investigated the expressed number of CR1 on erythrocytes (E) in relationship of the CR1 density genotype from 46 patients with systemic lupus erythematosus (SLE) and 47 healthy volunteers. The CR1 genotype was determined by a method based on polymerase chain reaction (PCR) amplification of the genomic DNA fragment of 1.8 kb separated byHindIIIendonuclease digestion and agarose gel electrophoresis. Our data supported the earlier results that the number of binding sites/E for monoclonal anti-CR1 decreased among SLE patients compared with normal individuals having the same alleles for the CRUE density. At the same time the novelty of our recent results was that the decreased expression of CR1 on E correlated significantly with kidney involvement in patients homozygous for the CR1/E high density allele (HH). These data suggest that the deficiency of the detectable number of CR1 on erythrocytes is acquired in this SLE population.
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