Long-run monitoring of bacteria, yeasts and other micromycetes in the air of an industrial conurbation
作者:
Jòsef Hýsek,
Zdeněk Fišar,
Bedřic Binek,
期刊:
Grana
(Taylor Available online 1991)
卷期:
Volume 30,
issue 2
页码: 450-453
ISSN:0017-3134
年代: 1991
DOI:10.1080/00173139109432007
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Three different methods were used for the monitoring of airborne microorganisms: (1). Cultivation of microbes trapped in a single-stage biological impactor directly on a solid agar nutrient medium (meat-pepton agar, Sabouraud's agar, blood agar) in Petri dishes. The repeated yearly course of concentrations of cultivable organisms, or colony-forming units (CFU), was obtained by long-run measurements. (2) Aeresol was trapped by impact on membrane filters, and the microorganisms were cultivated by placing the filters on the agar media as above. (3) Direct microorganism counting in a fluorescence microscope; air was sampled in a four-stage impactor where the aerosol was trapped on microscope slides, and the microorganisms were subsequently stained with fluorescent dyes (fluorescein diacctate, 4;6-diamidino-2-phenylindole and, particular, ethidium bromide).
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