Some aspects of quantification of neutral steroid metabolites in body fluids of healthy and uremic subjects by capillary gas chromatography
作者:
H. Ludwig‐Koehn,
H. V. Henning,
期刊:
Journal of High Resolution Chromatography
(WILEY Available online 1986)
卷期:
Volume 9,
issue 1
页码: 35-38
ISSN:0935-6304
年代: 1986
DOI:10.1002/jhrc.1240090106
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Gas chromatography, GC;Fused silica capillary columns;Quantification;Uremia;Steroid metabolites
数据来源: WILEY
摘要:
AbstractNeutral steroid metabolites enriched from urine and hemofiltrate were identified by gas chromatography/mass spectrometry and quantified by capillary gas chromatography. This study included 20 healthy controls and 37 uremic patients. Before enrichment of steroids from biological material, the standard deviation of the workup procedure and subsequent derivatization into the trimethylsilyl‐enol‐trimethylsilyl ethers was tested and found to be 2–5% in urine and 12–17% in the more complicated workup procedure of hemofiltrate, but essentially smaller than the biological standard deviation. Compared to the 24 h urinary excretion rates of controls, the excretion rates of androsterone, etiocholanolone, and corticoid metabolites were significantly lower in uremic body fluids, while those of 11‐oxygenated androstanolones, degradation products of corticoids, were enhanced in uremic urine. The ratio of corticoid metabolites to 11‐oxygenated androstanolones in urine of nondialyzed uremics correlated significantly with their plasma creati
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