The glycosylated hemoglobin (glyco Hb) and the glycoprotein of the plasma were determined using affinity chromatographic techniques in cord blood and maternal blood of normal (Group I), White's Class A diabetic (Group II), and insulin-dependent diabetic (Group III) mothers. The results revealed significant increase of glyco Hb (P< 0.005) and glycoprotein (P< 0.005) in the newborns of Group III mothers in comparison to those of Group I. The glyco Hb of maternal blood in, this group also showed significant increase (P< 0.005) while the increase in Hb A, and plasma glycoprotein were nonsignificant. AH values for cord blood and maternal blood of White's Class A diabetics revealed no significant change from the normal group. We conclude that the fetuses of insulin-dependent diabetic mothers are hyperglycemicin utero.