A micropropagation process was developed forAnogeissus latifolia-atree of fragile ecosystems. Multiple shoots were regenerated from cotyledonary node and epicotyl explants on Murashige and Skoog's (MS) medium containing 0.1 mgl-1indole-3-acetic acid (IAA) + 1.5 mgl-l6-benzylaminopurine (BAP) + additives (25 mgl-leach of adenine sulphate, L-arginine, ascorbic-, citric acids and 1.0 mM L-asparagine) + 200 μM Fe-EDTA (ferric-ethylenediaminetetraacetic acid) salt. The shoots differentiatedin vitrowere subcultured and repeatedly transferred onto fresh medium but with 1.0 mgl-1of BAP to achieve 4-5 fold rate of shoot multiplication. After every 4th week of culture, from each culture bottle 8-10 shoots could be harvested for rooting. The shoots produced in culture were rootedin vitroon half strength MS medium with 1.0 mgl-leither ofIBA(indolebutyric acid) or NAA (naphthaleneacetic acid). The shoots were pulse treated with combination (100 mgl-leach) ofIBAand NOA (2-naphthoxy acetic acid) rootedex vitro.Theex vitroroot induction method is highly efficient and plantlets so generated could be acclimatized and pot transferred. The process developed can be used for large scale production of plants of A.