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Optimal Conditions for Demonstrating Human Lymphocyte Differentiation Antigens: A Working Protocol for the Immunohistochemical Analysis of a Lymph Node Biopsy

 

作者: EliasJules M.,   La NeveDebra,   BeutnerErnst H.,   HogrefeWayne,   KatelyJohn,   BrittenTom,   RoussoCarmen,  

 

期刊: Journal of Histotechnology  （Taylor Available online 1985）
卷期: Volume 8, issue 4  

页码: 201-203

 

ISSN:0147-8885

 

年代: 1985

 

DOI:10.1179/his.1985.8.4.201

 

出版商: Taylor&Francis

 

数据来源: Taylor

 

摘要:

AbstractThe histologic requirements for preservation of lymphocyte membrane antigens were discussed by the participants of a conference on leukemia and lymphoma. The risk of false negative staining is minimized if surgical lymph node biopsy specimens are processed promptly into OCT and quick-frozen with liquid nitrogen. Cut sections must be adequately dried and fixed in“dry”acetone.

 

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