Improved and Rapid Liquid Chromatographic Determination of Indomethacin in Serum
作者:
H.W. Jun,
Harry Suryakusuma,
期刊:
Analytical Letters
(Taylor Available online 1982)
卷期:
Volume 15,
issue 13
页码: 1063-1071
ISSN:0003-2719
年代: 1982
DOI:10.1080/00032718208064420
出版商: Taylor & Francis Group
关键词: Indomethacin;Analysis;LC;Dog;Serum Levels
数据来源: Taylor
摘要:
A rapid, specific and sensitive reversed-phase liquid chromatographic (LC) assay for the quantitative determination of indomethacin in serum without extraction was developed. Chromatographic separation using flunixin meglumine as the internal standard was achieved on octadecylsilane-coated particles with a mobile phase of 0.15 M acetate buffer pH 3.0 (50% v/v), acetonitrile (30% v/v) and methanol (20% v/v). The recovery of indomethacin from serum samples in the concentration range of 0.1-25 μg/ml was 95.5 ± 5.8% and as little as 100 ng/ml of indomethacin in serum samples can be quantitated by this procedure. A serum level versus time profile of dog with intravenously administered indomethacin demonstrated the applicability of the assay.
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